Anti-oxidant and Anti-inflammatory Activity of Ethyl Acetate Fraction of Moringa oleifera Flowers

Main Article Content

A. Rajeshkanna
M. M. Senthamilselvi
D. Prabhakaran


Aims: To investigate the antioxidant and anti-inflammatory activities of ethyl acetate extract of  Moringa oleifera flowers.

Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between April 2017 and January 2018.

Methodology: Extraction and fractionation were carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate.  The anti-inflammatory effect of the extract was investigated by HRBC membrane stabilization and Albumin denaturation methods. Anti-oxidant effect of the extract was determined by DPPH assay and ABTS method.

Results: The dry sample extracted from the ethyl acetate fraction of Moringa oleifera flowers possess highly anti-oxidant activity showed by the DPPH assay and ABTS method and also having anti-inflammatory activity is determined by human red blood cell (HRBC) membrane stabilization and Albumin denaturation methods. However, these effects need to be confirmed using in vivo models and clinical trials before its utilization as a therapeutic agent.

Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Moringa oleifera flowers possesses effective anti-oxidant and anti-inflammatory activities.

ABTS assay, anti-inflammatory activity, antioxidant activity, HRBC method, Moringa oleifera.

Article Details

How to Cite
Rajeshkanna, A., M. Senthamilselvi, M., & Prabhakaran, D. (2020). Anti-oxidant and Anti-inflammatory Activity of Ethyl Acetate Fraction of Moringa oleifera Flowers. European Journal of Medicinal Plants, 30(4), 1-8.
Original Research Article


Boonchum W, Peerapornpisal Y, Vacharapiyasophon P, Pekkoh J, Pumas C, Jamjai U, et al. Antioxidant activity of some seaweed from the gulf of Thailand. Int J Agric Biol. 2011;13:95–99.

Pakade V, Cukrowska E, Chimuka L. Comparison of antioxidant activity of Moringa oleifera and selected vegetables in South Africa. South African Journal of Science. 2013;109:1-5

Moon J, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009;57:1655–1666.

Laandrault N, Pouchert P, Ravel P, Gase F, Cros G, Teissedro PL. Antioxidant activities and phenolic level of French wines from different varieties and vintages. J Agric Food Chem. 2001;49:3341–3343.

Mukazayire MJ, Allaeys V, Duez P, Calderon PB, Stevigny C, Bigendako MJ. Evaluation of the hepatotoxic and hepatoprotective effect of Rwandese herbal drugs on in vivo (Guinea pigs barbiturate-induced sleeping time) and in vitro (rat precision-cut liver slices, PCLS) models. Experimental and Toxicologic Pathology. 2010;62(3):289–299.

Marletta MA. Nitric oxide syntheses structure and mechanism. J. Biol. Chem. 1993;268:12231–12234.

Watkins LR, Milligan ED, Maier SF, Glial proinflammatory cytokines mediate exaggerated pain states: Implications for clinical pain. Advances in Experimental Medicine and Biology. 2003;521:1–21.

Smejkal K. Selected natural phenolic compounds - potential treatment for peripheral neuropathy? Ceska Slovenska Farmacie. 2014;63:55–70.

Nadkarni KM, Nadkarni AK Chopra RN. The Indian Materia Medica. Popular Prakasan, Bombay. 1954;1:811 – 816.

Fahey JW. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Tree for Life Journal. 2005;1:5-20.

Torres-Castillo JA, Sinagawa-Garcia SR, Martinez-Avila GCG, Lopez-Flores AB, Sanchez-Gonzalez EI, Aguirre-Arzola VE, Torres-Acosta RI, Olivares-Saenz E, Osorio-Hernandez E, Gutierrez-Diez A. Moringa oleifera phytochemical detection, antioxidants, enzymes and antifugal Properties. Phyton International journal of experimental botony. 2013;82:193-202.

Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research. 2007;21:17-25.

Goyal, BR, Agrawal BB, Goyal RK Mehta AA. Phyto-pharmacology of Moringa oleífera Lam. An overview. Natural Product Radiance. 2007;4:347-353.

Chumark P, Khunawat Y, Sanvarinda S, Phornchirasilp N, Phumala Morales L, Phivthongngam P, Ratanachamnong S, Srisawat, Pongrapeeporn KS. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. Journal of Ethnopharmacology. 2008;116:439-446.

Peixoto JR, Silva GC, Costa RA, De Sousa Fontenell JR, Vieira GH, Filho AA, Dos Fernandes Vieira RH. In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts. Asian Pacific Journal or Tropical Medicine. 2011; 4:201-204.

Kooltheat, N, Sranujit, R, Chumark P, Potup P, Laytragoon-Lewin N, Usuwanthim K. An ethyl acetate fraction of Moringa oleifera Lam. inhibits human macrophage cytokine production induced by cigarette smoke. Nutrients, 2014;6(2):697-710.

Solomon S, et al, Anti-oxidant and anti-inflammatory activity of Cascabela thevetia (Flowers), Indo Am. J. P. Sci. 2016;3(6).

Ilavarsan R, Mallika M, Venkataraman S. Anti-inflammatory and antioxidant activities of cassia fistula linn bark extracts. African Journal of Traditional, Complementary and Alternative Medicines. 2005;2(1):70-85.

Roberta Re, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang, Catherine Rice-Evans. Antioxidant Activity Applying An Improved Abts Radical Cation Decolorization Assay. Free Radical Biology & Medicine. 1999;26:1231–1237.

Gopalakrishnan S, Vadivel E, Dhanalakshmi K. Anti-inflammatory and analgesic activities of Tephrosia purpurea Linn. aerial and root extracts. Journal of Pharmacy Research 2010;3:1103-1106.

Muruganantham N, Solomon S, Senthamilselvi MM. Anti-oxidant and Anti-inflammatory activity of Cucumis sativas (Cucumber) Flowers. Int J Pharm Sci Res 2016;7(4):1740-45.

Nagaharika Y, kalyani V, Rasheed S and karthikeyan R. Anti inflammatory activity of leaves of Jatropha gossypifolia L. by hrbc membrane stabilization method. Journal of Acute Disease. 2013;156-158.

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences. 2010;146-155.

Brown JH, Mackey HK. Inhibition of heat-induced denaturation of serum proteins by mixtures of nonsteroidal anti-inflammatory agents and aminoacids. Proceedings of the Society for Experimental Biology and Medicine. 1968;128:225-8.

Solomon S, Murugananthan N, Senthamilselvi MM. Anti-oxidant and anti-inflammatory activity of Thespesia populnea (flowers). Pharmacophore. 2015; 6(1):53-59.