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The present study was aimed to investigate the histochemical and phytochemical studies of four important medicinal plants. Leaf, stem and root sections were examined for alkaloid, protein and phenolic tests. Methanolic extracts of leaf, stem and root were analysed for the phytochemical screening of major bioactive constituents. Histochemical analysis reveals the presence of alkaloids, phenols and proteins in epidermis, cortex and vascular bundles of root, stem and leaf. Preliminary phytochemical analysis showed the presence of alkaloids, tannins, phenols, flavonoids, steroids, saponins, terpenoids, coumarins, quinone and proteins. The root extract of Catharanthus roseus recorded maximum content of phenols compared to other extracts. Costus pictus root and leaf extracts exhibited a significant amount of flavonoids and tannins respectively. Further studies were focused on isolation and characterization of each plant compounds.
Baris O, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, et al. Turkish Journal of Biology. 2006;30:65-73.
Ncube N, Okoh SA, Afolayan AI. African Journal of Biotechnology. 2008;7(12): 1797-1806.
Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in virgin olive oil. International Journal of Molecular Sciences. 2010;11(2):458-79.
Nicholson RL, Hammerschmidt R. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology. 1992;30:369-389.
Khare CP. Alpinia galanga – An important medicinal plant: A review. A Dictionary of Indian Medicinal Plant, Published by Springer India Pvt. Ltd. 2007;37.
Yang X, Eilerman RG. Pungent principle of Alpinia galanga (L.) Swartz and its applications. Journal of Agricultural and Food Chemistry. 1999;47(4):1657-1662.
Mori HK, Kubota, Kobayashi A. A potent aroma components of rhizomes from Alpinia galangal. Nippon Shokuhin Kagaku Kogaku Kaishi. 1995;42:989-995.
Zheng GQ, Kenney PM, Lam LKT. Potential anticarcinogenic natural products. Journal of Agricultural and Food Chemistry. 1993;41(2):153-156.
Itokawa HH, Morita T, Sumitomo N, Totsukaand K, Takeya. Antitumor principles from Alpinia galanga. Planta Medica. 1987;53:32-33.
Sarin YK, Singh A, Bedi KL, Kapur SK, Kapahi BK, Atal CK. Costus speciosus rhizomes as a commercial source of diosgenin. Cultivation and Utilization of Medicinal Plants, C. K. Atal and B.M. Kapur (Eds.), RRL-CSIR, Jammu Tawi. 1982;106-118.
Gupta AK, Tondon N, Sharma M. Quality standards of Indian medicinal plants, Medicinal Plants Unit. Published by Indian Council of Medical Research. 2008;7: 48.
Lata B. Phytochemical Rev. 2007;6:403-411.
Sain M, Sharma V. Catharanthus roseus (An anti-cancerous drug yielding plant) - A review of potential therapeutic properties. International Journal of Pure Applied Biosciences. 2013;1(6):139-142.
Kumar A, Singhal C, Sharma RA, Vyas GK, Kumar V. Anaaysis of antioxidant activity of Catharanthus roseus L. and its association with habitat tempaerature. Asian Journal of Experimental Biological Sciences. 2012;3(4):706-713.
Malik AA, Showkat RM, Ahmad J. Ruta graveolens essential oil composition under different nutritional treatments. American-Eurasian Journal of Agricultural & Environmental Sciences. 2013;13(10):1390-1395.
Kuster VC, Vale FH. Leaf Histochemistry analysis of four medicinal species from Cerrado. Revista Brasileira De Farmacognosia. 2016;26:673-678.
Dhanapal ACTN, Ming TW, Aung HP, Hao SJ. Preliminary screening of Artemisia argyi for antioxidant potentials. International Journal of Pharmacognosy and Phytochemical Research. 2016;8:347-355.
Harbone JB. Phytochemical methods. Chapman and Hall Ltd, London. 1973;33-80.
Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry. 2003;81(3):321-326.
Embrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. African Journal of Biotechnology. 2008;7(18):3188-92.
Medini F, Fellah H, Ksouri R, Abdelly C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science. 2014;8:216-224.
Dhale DA. Histochemical investigation of some medicinal plants. Advance Research in Pharmaceuticals and Biologicals. 2011;1(2):147-154.
Jing H, Liu J, Liu H, Xin H. Histochemical investigation and kinds of alkaloids in leaves of different development stages in Thymus quinquecostatus. The Scientific World Journal. 2014;1-6.
Kadam VB, Famila S, Tambe SS, Momin. Histochemical investigation of different organs of two medicinal plants in Maharashtra. International Journal of Pharmaceutical Research and Bioscience. 2013;2(4):194-201.
Singh, Singh. Preliminary phytochemical screening and determination from rhizome and flower of Alpinia galanga. World Journal of Pharmacy and Pharmaceutical Sciences. 2014;3(11):1354-1361.
Subash KR, Bhaarathi MG, Rao JN, Cheriyan BV. Phytochemical screening and acute toxicity study of ethanolic extract of Alpinia galanga in rodents. International Journal of Medical Research and Health Sciences. 2012;2(1):93-100.
Malleshwari D, Kausar R, Bagyanarayana G. Primary screening of phytochemicals present in crude extracts of leaf, stem and rhizome of Costus igneus (Insulin plant). International Journal of Research in Advent Technology. 2019;7(3):1352-1357.
Meshram PV, Moregonak SD, Gatne MM, Gaikwad RV, Zende RJ, Ingole SD, Vanage GR. Physicochemical and phytochemical screening of aqueous and ethanolic extracts of Costus pictus D. Don and Enicostema littorale blim. Chemical Science Review and Letters. 2017;6(21):426-434.
Paikara D, Pandey B, Singh S. Phytochemical analysis and antimicrobial activity of Catharanthus roseus. Indian Journal of Scientific Research. 2017;12(2):124-127.
Kannabiran K, Rajamani K, Suresh J, Joel RJ, Uma D. Variability in alkaloid content and phytochemical profile of periwinkle (Catharanthus roseus) cv. local through gamma and ethyl methane sulphonate. Journal of Pharmacognosy and Phytochemistry. 2017;6(5):2528-2532.
Renugadevi V, Meerabai RS. Phytochemical analysis and antimicrobial activity of leaf extract of Ruta graveolens L. Journal of International Academic Research for Multidiscipinary. 2016;4(3): 57-65.
Malik, Pandey DK, Okram A. Evaluation of phytochemicals, antioxidants, antibacterial and diabetic potential of Alpinia galanga and Eryngium foetidum plants of Manipur (India). Pharmacognosy Journal. 2016;8(5):459-464.
Melanathuru V, Rengarajan S, Thangavel, N. Comparative study of antioxidant and anticancer activity of Alpinia calcarata and Alpinia galanga. International Journal of Pharmacy and Pharmaceutical Sciences. 2017;9(12):186-193.
Ramya R, Dhamodharan R. Qualitative and quantitative analysis of phytochemicals of Costus speciosus. International Journal of Health Science and Research. 2015;5(12):170-176.
Kabesh K, Senthikumar P, Ragunathan R, Raj Kumar R. Phytochemical analysis of Catharanthus roseus plant extract and its antimicrobial activity. International Journal of Pure and Applied Bioscience. 2015;3(2):162-172.
Azlework HG, Sahabjada, Jafri A, Arshad MD, Malik T. Phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens L. from Ethiopia. International Journal of Pharmaceutical Sciences. 2017;9(6):1- 5.
Ahmed MA, Edrah S, Kahal F. Phytochemical screening, antioxidant activity and total phenols of “Sonchus oleraceus asteraceae” and Ruta graveolens” from Libya. International Journal of Scientific Engineering and Research. 2015;5(4):61-66.
Muthukumar C, Cathrine L, Gurup S. Qualitative and quantitative phytochemical analysis of Costus igenus leaf extract. Journal of Pharmacognosy and Phytochemistry. 2019;8(4):1595-1598.
Nayak S, Babu G. Phytochemical studies on Catharanthus roseus grown in eco-friendly feather compost. Journal of Pharmacognosy and Phytochemistry. 2015;4(4):169-174.
Benazir JF, Suganthi R, Devi R, Suganya K, Monisha K, Nizar Ahamed KP, Santhi R. Phytochemical profiling, antimicrobial and cytotoxicity studies of methanolic extracts from Ruta graveolens. Journal of Pharmacy Research. 2011;4(5):1407-1409.
Al-Enazi. Phytochemical screening and biological activities of some species of Alpinia and Convolvulus plants. International Journal of Pharmacology. 2018;14(3):301-309.
Chibueze UF, Kingsley AN, Okorie NK, Akuagwu A, Success N. Phytochemical composition of Costus afer extract and its alleviation of carbon tetrachloride – induced hepatic oxidative stress and toxicity. International Journal of Modern Botany. 2012;2(5):120-126.
Uadia RN, Ugwu I, Erameh T, Osunde E. Estimation of tannins, alkaloids, saponins and proximate composition of Vernonia amygdalina (Del) root. International Journal of Herbal Medicine. 2017;5(3):88-92.