Main Article Content
Fruits are highly nutritious, sources of vitamins, minerals, fibers etc. and these are part of our daily diet. However, during cultivation, harvesting, transportation, handling fruits get contaminated with pathogenic microorganisms which leads to severe problems to community. Owning to this point, in current research, bacteriological analysis was performed on Sofeda, Pineapple, Grape, Banana, Apple, Orange, Guava, papaya, Jujube and Starfruit. Total 50 samples were randomly collected from market and street vendors of Dhaka city. Higher numbers of rotten fruits were present in wholesale markets. 35 strains were isolated which included Salmonella spp., Acinetobacter spp., Klebsiella spp., Vibrio cholerae, Vibrio parahaemolyticus, Staphylocoous aureus and E. coli. They were identified using biochemical test and antibiogram for selective isolates. In case of drug resistance of isolates, majority exhibited resistance against Erythromycin, Vancomycin and Amoxycillin and showing sensitivity against Ciprofloxacin and Ceftriaxone. It was observed in the current study that 100% isolates were resistant against Erythromycin, followed by Amoxycillin 90.63% and vancomycin 86.25%, where only 35.27% isolates were resistant against Ciprofloxacin. In case of sensitivity 64.73% isolates were sensitive against Ciprofloxacin followed by Ceftriaxone 66.25%.
Rawat S. Food spoilage: Microorganisms and their prevention. Asian Journal of Plant Science and Research. 2015;5(4): 47-56.
Bhale UN. Survey of market storage diseases of some important fruits of Osmannabad District (M.S.). India Science Research Reporter. 2011;1(2):88-91.
Akinmusire OO. Fungal species associated with the spoilage of some edible fruits in Maiduguri Northern Eastern Nigeria. Advances in Environmental Biology. 2011; 5(1):157-161.
Tournas VH, Katsoudas E. Mould and yeast flora in fresh berries, grapes and citrus fruits. International Journal of Food Microbiology. 2015;105(1):11-17.
Mohammad Al-Masum Molla. Fruits for all seasons. The daily Star; 2019.
(Accessed 25 November 2019)
Nahar Q, Choudhury S, Faruque O, Saliheen SSS, Siddiquee MA. Dietary Guidelines for Bangladesh. 2013:2-5.
Lack of Post-harvest Handling Knowledge - Tonnes of vegetables, fruits go to waste annually. The Daily Star; 2019.
(Accessed 25 November 2019)
Rolle RS. Improving postharvest management and marketing in the Asia-Pacific regions: Postharvest Management of Fruits and Vegetables in Asia-Pacific Region. FAO (Food and Agricultural Organizations) and APO (Asian Productivity Organization). 2006;23-31.
Senjuti JD, Feroz F, Tahera J, Das KK, Noor R. Assessment of microbiological contamination and the In vitro demonstration of the anti-bacterial traits of the commonly available local fruit blend within Dhaka Metropolis. Journal of Pharmacognosy and Phytochemistry. 2014;3(1):73-77.
Quaiyum S, Tanu NI, Sharmin M, Paul L, Munna S, Das KK, Acharjee, Noor R. Microbiological contamination and anti-bacterial traits of common oral herbal medicinal products within Dhaka Metropolis. European Journal of Medicinal Plants. 2014;4(7):872-881.
Jelodarian S, Ebrahimabadi SA, Kashi FJ. Evaluation of antimicrobial activity of Malus domestica fruit extract from Kashan area. Avicenna Journal of Phytomedicine. 2013; 3(1):1-6.
Tahera J, Feroz F, Senjuti JD, Das KK and Noor R. Demonstration of anti-bacterial activity of commonly available fruit extracts in Dhaka, Bangladesh. American Journal of Microbiological Research. 2014;2(2):68-73.
Feroz F, Senjuti DJ ,Noor R. Determination of microbial growth and survival in salad vegetables through In vitro challenge test. International Journal of Nutrition and Food Sciences. 2013;2(6): 312-319.
Rahman F, Noor R. Prevalence of pathogenic bacteria in common salad vegetables of Dhaka Metropolis. Bangladesh Journal of Botany 2012;41 (2):159-162.
Abadias M, Usall J, Anquera M, Solsona C, Vinas I. Microbiological quality of fresh, minimally processed fruit and vegetables, and sprouts from retail establishments. International Journal of Food Microbiology, 2008;123(1-2):121-129.
Walker M, Phillips CA. The effect of preservatives on Alicyclobacillus acid-oterrestris and Propionicacterium cyclohexanicum in fruit juices. Food Control. 2008;19(10):974-981.
Lund B, Snowdon A. The microbiological safety and quality of food. Fresh and Processed Food. 2000;1:738-58.
Banik A, Abony M, Datta S, Towhid ST. Microbial status and multidrug resistance pattern of pathogenic bacteria isolated from street food in Dhaka City, Bangladesh. Journal of Advances in Microbiology. 2018;13(1):1-13.
Banik A, Abony M, Datta S, Towhid ST. Microbiological quality of ready-to-eat food from Dhaka, Bangladesh, Current Research in Nutrition and Food Science. 2019;07(1):161-168.
Clauss D, Berkeley RCW, Genus Bacillus in Bergey’s manual of determinative bacteriology, Sneath PH a Baltimore. MD: Williams Wilkins. 1986;2:1105.
Flores O, Belanche LA, Blanch AR. New multiplatform computer program for numerical identification of microorganisms. Journal of Clinical Microbiology. 2009; 47:4133-4135.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1966; 45(4):493–496.
Modak B, Rahman S, Gazi MS, Uddin A, Rahman T. Antimicrobial potency assay of common antibiotics collected from different drug stores in Dhaka Metropolis. Stamford Journal of Microbiology. 2013;3(1):26-29.
Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). Performance standards for antimicrobial susceptibility testing. 25th Informational Supplement Document M100-S17: 1. Wayne, Pennsylvania. 2015;32-80.
Akter R, Tanu NI, Uddin MA.Isolation and quantification of bacterial isolates from citrus fruits and determination of their anti-bacterial activity against selected pathogens. Stamford Journal of Microbiology. 2015;3(1):30–33.
Butt AA, Aldridge KE, Sanders CV. (2004) Infections related to the ingestion of seafood. Part II: Parasitic infections and food safety. Lancet Infectious Diseases. 2004;4(5):294-300.
Kasture N. Bacteriological analysis of fresh vegetables, fruits from local market. International Journal of Fauna and Biological Studies. 2017;4(6):59-61.
Kumar A, Bhushan V, Verma S, Srivastav G, Kumar S. Isolation and characterization of microorganisms responsible for different types of food spoilages. International Journal of Research in Pure and Applied Microbiology. 2011;1(2):22- 31.
Hasan NA, Zulkahar IM. Isolation and identification of bacteria from spoiled fruits. AIP Conference Proceedings. 2018;2020 (1):020073.
Tasnim FH. Quality assessment of industrially processed fruit juices available in Dhaka City, Bangladesh. Malaysian Journal of Nutrition. 2010;16(3):431-438.
Hemalata VB, Virupakshaiah DBM. Isolation and identification of food borne pathogens from spoiled food samples. International Journal of Current Microbiology and Applied Sciences. 2016; 5(6):1017-1025.
Mamun SA, DAS KK, Uddin A. Microbiological analysis and determination of antibacterial activity of apple samples collected from local markets in Dhaka city, Bangladesh. Stamford Journal of Microbiology. 2016;6(1):11-15.
Kamal Rai Aneja, Romika Dhiman, Neeraj Kumar Aggarwal, Vikas Kumar and Manpreeet Kaur. Microbes associated with freshly prepared juices of citrus and carrots. International Journal of Food Science; 2014.