Chemical Constituents from Wood of Zanthoxylum rigidum Hum. & Bonpl. Ex Willd (Rutaceae) and Antileishmanial Activity
Carlos H. C. dos Santos
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
Mayara C. Sousa
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
Paulo T. de Sousa Jr.
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
Tereza A. N. Ribeiro
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
Marco J. Jacinto
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
Virgínia C. Paulino Silva *
Department of Chemistry, Federal University of Mato Grosso, Mato Grosso, Cuiabá, Brazil.
*Author to whom correspondence should be addressed.
Abstract
In this study was reported the structural elucidation by means of nuclear magnetic resonance (1D and 2D), FT-IR and mass spectrometry of lupeol (1), a mixture of the steroids campesterol (2), b-sitosterol (3) and stigmasterol (4), sesamin (5), trans-dimethylmatairesinol (6), trans-methylpluviatolide (7), quercetin (8), and d-mannitol (9) isolated from wood of Zanthoxylum rigidum. The lignans 5, 6 and 7 were tested for their ability to inhibit the proliferation of the promastigote forms of Leishmania braziliensis and Leishmania chagasi. Only sesamin (5) and trans-methylpluviatolide (7) showed moderate inhibitory activity with IC50 = 22.4 and 33.6, SI = 3.87 and 2.41 and CC50 = 86.9 and 81.2, for L. braziliensis and L. chagasi, respectively, compared to the positive control (amphotericin B).
Keywords: Zanthoxylum rigidum, antileishmanial, Leishmania braziliensis, Leishmania chagasi, NMR, MS