Evaluation of Physiochemical Properties, Thermal Behavior and Phytopharmaceutical Potential of Citrus aurantium’s Essential Oil
Vikas Jha *
National Facility for Biopharmaceuticals, Guru Nanak Khalsa College, Mumbai - 400019, India.
Fatimabi Shaikh
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Nikita Tendulkar
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Siddhartha Pandya
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Bhakti Madaye
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Esha Gupta
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Farheen Khan
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Navdeep Kaur
National Facility for Biopharmaceuticals, Guru Nanak Khalsa College, Mumbai - 400019, India.
Tisha Jain
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai - 19, India.
Stanford Almeida
Department of Biotechnology, St. Xavier's College Autonomous, Mumbai - 400001, India.
Pratyusha Mane
Department of Biotechnology, Guru Nanak Khalsa College, Mumbai - 400019, India.
*Author to whom correspondence should be addressed.
Abstract
The study investigates the therapeutic potential of the Citrus aurantium var. amara essential oil extracted from the blossoms of the bitter orange plant by examining its chemical composition, thermal stability, and potency against infectious disease-causing pathogens. Initially, the volatile components of the essential oil were evaluated by obtaining a chromatographic fingerprint using HPTLC and FTIR spectrum identification. Furthermore, a thermal profile of the essential oil was obtained using the thermogravimetric-differential thermal analysis and differential scanning calorimetric analysis. A predetermined set of antibiotic-resistant microorganisms were used to examine the antibacterial activity of the essential oil. Lastly, its anti-inflammatory activity was assessed using the albumin denaturation assay. The research concluded that the Citrus aurantium var. Amara essential oil exhibits potential therapeutic characteristics which can be further explored through in vivo studies.
Keywords: Antibacterial, anti-inflammatory, antibiotic-resistance, citrus aurantium var. amara, differential scanning calorimetry, essential oil, fourier-transform infrared spectrometry, high-performance thin layer chromatography, thermogravimetric-differential thermal analysis