Organogenesis of Abelmoschus moschatus Medik. Using Aseptic Seedling Explants
Aruna Varimadugu *
Department of Biotechnology, Chaitanya Bharathi Institute of Technology, Hyderabad, Telangana, India
Pullaiah Thammineni
Department of Botany, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India
*Author to whom correspondence should be addressed.
Abstract
Aim: Abelmoschus moschatus have been extensively used in traditional medicine as well as in perfume industries. The primary goal of the present research was to develop an efficient plant regeneration protocol of Abelmoschus moschatus from aseptic seedling explants such as cotyledon, internode, leaf and root.
Methodology: The seeds of Abelmoschus moschatus were surface sterilized with 0.1% mercuric chloride and 70% ethanol were cultured on ½ MS basal media for developing aseptic seedlings Aseptic seedling explants were cultured on different concentrations of auxins for callus induction. Later callus was transferred on to different concentrations of cytokinins for shoot regeneration and for in vitro, rooting different concentrations of auxins were used. Finally, such in vitro developed plantlets were acclimatized.
Results: Half strength MS medium with 1% sucrose was used for raising aseptic seedlings. Maximum of 92% response of callus induction was obtained from leaf explants on MS medium + 2 mg/L 2, 4-dichlorophenoxyacetic acid. An average of 2.4 shoots per callus were observed on MS + 2 mg/L benzyl-6-aminopurine from leaf explant. The regenerated shoots were best rooted on 1/2 MS + 0.5 mg/L indole-3-butyric acid. The regenerated plantlets were established with 70% survival.
Conclusion: An efficient plant regeneration protocol of Abelmoschus moschatus was developed.
Keywords: Abelmoschus moschatus, callus, leaf explant, shoot regeneration