Phytochemical Screening, Anti-radical and Antibacterial Activities of Leaves from Combretum glutinosum, a Plant Used in Treatment of Urinary Infections in Benin Republic
Emmanuel O.D. BAKROU *
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
Yaya Alain KOUDORO
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
Durand DAH-NOUVLESSOUNON
Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology, University of Abomey-Calavi, 05BP:1604, Cotonou, Benin.
Cokou Pascal AGBANGNAN DOSSA
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
Alain Guy ALITONOU
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
Lamine BABA-MOUSSA
Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology, University of Abomey-Calavi, 05BP:1604, Cotonou, Benin.
Dominique C. K. SOHOUNHLOUE
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
Félicien AVLESSI
Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin.
*Author to whom correspondence should be addressed.
Abstract
Infectious diseases are a major global health problem. Bacterial infections represent about 70% of mortality cases. This work aims to evaluate Combretum glutinosum through identification of secondary metabolites and evaluation of anti-radical and antibacterial activities of its leaves. Secondary leaf metabolites of Combretum glutinosum were identified by specific staining and precipitation reactions for each metabolite before phenolic compounds were determined by spectrophotometer. Antibacterial activity was assessed by macrodilution method in microplates and Petri dishes. Based on results obtained, Combretum glutinosum leaves contain a diversity of secondary metabolites with a total flavonoid content of 16.78 mgEQ/gEx, total phenols of 93.291 mgEAG/gEx and total tannins of 0.583 mgEL/g Ex. Hydroethanolic extract of C. glutinosum leaves showed bactericidal activity against strains of Staphylococcus aureus and Streptococcus D, Klebsiella pneumoniae and Escherichia coli. There is a gradual increase in the rate of DPPH radical capture with increasing concentration of Combretum glutinosum hydroethanolic extract. Thus the lowest IC50 (3.5mg/mL) was obtained with hydroethanolic extract of Combretum glutinosum. These findings support the traditional use of Combretum glutinosum in treating urinary infections and suggest its potential as a source of antibacterial and antioxidant agents.
Keywords: Infectious diseases, Combretum glutinosum, secondary metabolites, bactericidal, anti-radical