Effects of Exogenous Growth Regulators on Direct and Indirect Micropropagation of Corbichonia decumbens (Forssk.) Exell (Molluginaceae)
G. Uma
PG and Research Department of Botany, Kongunadu Arts and Science College (Autonomous), Coimbatore-29, Tamil Nadu, India
R. Prabakaran
Department of Botany, PSG College of Arts and Science, Coimbatore-641014, India
K. Kalimuthu *
PG and Research Department of Botany, Plant Tissue Culture Division, Government Arts College (Autonomous), Coimbatore-641018, India
V. Chinnadurai
PG and Research Department of Botany, Plant Tissue Culture Division, Government Arts College (Autonomous), Coimbatore-641018, India
V. Balasubramaniam
PG and Research Department of Botany, Kongunadu Arts and Science College (Autonomous), Coimbatore-29, Tamil Nadu, India
*Author to whom correspondence should be addressed.
Abstract
Corbichonia decumbens (Forssk.) Exell is a medicinally important plant belonging to the family Molluginaceae. In this study we have standardized a protocol for both direct and indirect micropropagation. Callus was induced from the leaf, node and internode explants on Murashige and Skoog (MS) medium containing 6 benzylaminopurine (BAP 4.44 μM) in combination with naphthalene acetic acid (NAA 5.37 μM). 7.33 shoots were observed from callus while 32.16 shoots were observed during sub culture. In nodal culture 11.16, 34.16 number of shoots were obtained in initiation and subculture respectively on the MS medium supplemented with BAP (4.44 μM) and TDZ (2.27 μM). Regenerated shoots were rooted on MS medium along with NAA (16.05 μM) and activated charcoal (2 g/l). The rooted in vitro raised plantlets were acclimatized in shade house and successfully transferred to normal environment with higher survival rate (90.21%). The supplementation of growth hormones BAP and NAA enhanced the multiple shoot formation in direct and indirect micropropagation. Root formation is at the maximum in NAA and activated charcoal combination. Hence, the direct and indirect micropropagation of C. decumbens is more effective, reliable, reproducible and commercially viable than the conventional methods.
Keywords: Corbichonia decumbens, micropropagation, callus culture, acclimatization