Suppression of Inflammatory Mediators by the Ethanol Extract of Crotalaria verrucosa L. leaf – in vivo and in vitro Analysis
Kashfia Nawrin
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Mohammad Mustakim Billah
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Mohammed Sahek Ullah Jabed
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Mohammad Ibrahim Khalil
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Md. Main Uddin
Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Bangladesh
Md. Nurul Islam *
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
*Author to whom correspondence should be addressed.
Abstract
Objective: The present study demonstrated the potential of Crotalaria verrucosa L., a widely used medicinal plant of Bangladesh, ethanolic leaf extract in inhibiting the cascades of inflammation.
Methods: Carrageenan induced rat paw edema and xylene induced mice ear edema tests were performed as in vivo assessment of anti-inflammatory activity. Moreover, these methods were supported by the in vitro heat induced protein denaturation and haemolysis tests.
Results: In all experiments CVE extract shows moderate to significant efficacy. CVE 600 mg/kg significantly suppressed the biological response (edema) in comparison to both steroidal and non-steroidal anti-inflammatory drugs. Moreover in vitro study suggested that the leaf extract at doses of 300, 400 and 500 µg/ml possess moderate to high inhibition capacity for auto antigen production which exerts extract’s potential against Type III hypersensitivity.
Conclusion: All these data support the safe use of this plant traditionally. However, the extract is yet to be explored for responsible compounds and specific mechanisms.
Keywords: C. verrucosa, paw edema, ear edema, protein denaturation, haemolysis