Antioxidant and Anticonvulsant Effect of Dennettia Tripetala on Rat Model of Isoniazid-induced Seizure

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Elizabeth Finbarrs-Bello
Ignatius Ikemefuna Ozor
Samsudeen G. Ojuolape
Abonyi Ernest Obinna


Aims: This study aimed at investigating the antioxidative, anticonvulsive and histological effects of ethanolic fruit extract of Dennettia tripetala on the pre-frontal lobe of the brain in isoniazid-induced (300 mg/kg, i.p) seizure in adult wistar rat.

Introduction: Neuronal hyper-excitability and excessive production of free radicals have been implicated in the pathogenesis of a considerable range of neurological disorders, including epilepsy. The high rate of oxidative metabolism, coupled with the low antioxidant defenses and the richness in polyunsaturated fatty acids, makes the brain highly vulnerable to free radical damage.

Study Design: This is an original research conducted in Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology (ESUT), Parklane, Enugu State, Nigeria, between June and August, 2019.

Methodology: A total number of twenty four wistar rats were used for this experiment, the animals were grouped into six groups with four animals per group, Group I served as the negative control, Group II served as the positive control, Group III received the standard drug as well as the Isoniazid, while group IV,  V and VI  were treated with  ethanolic extract of Dennettia tripetala at different dosages; 250 mg/kg, 500 mg/kg and 750 mg/kg, intraperitoneally (i.p) respectively and its effects compared with a standard drug (Pyridoxine) treated group.

Results: The extract significantly prolonged the onset of seizure at high dose administration (750 mg/kg) but completely prevented seizure occurrence at low and medium dose administration (250 mg/kg and 500 mg/kg, i.p) when induced with isoniazid (300 mg/kg, i.p.).

Conclusion: The results obtained from this work suggest that ethanolic extract of Dennettia tripetala has anticonvulsant activity, and this supports the use of the formulation traditionally in the treatment of convulsions, thus should be considered for clinical trials.

Dennettia tripetala, antioxidant, anticonvulsant, seizure, ethanolic extract.

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How to Cite
Finbarrs-Bello, E., Ozor, I. I., G. Ojuolape, S., & Ernest Obinna, A. (2019). Antioxidant and Anticonvulsant Effect of Dennettia Tripetala on Rat Model of Isoniazid-induced Seizure. European Journal of Medicinal Plants, 30(2), 1-10.
Original Research Article


Devi PU, Manocha A, Vohora D. Seizures, antiepileptics, antioxidants and oxidative stress: An insight for researchers. Expert Opin Pharmacother. 2008;9(18):3169-77.
DOI: 10.1517/14656560802568230

Carlos Clayton Torres Aguiar, Anália Barbosa Almeida, Paulo Victor Pontes Araújo. Oxidative Medicine and Cellular Longevity. 2012;12.
Article ID 795259

Asehinde S, Ajayi A, Bakre A, Omorogbe O, Adebesin A, Umukoro S. Effects of Jobelyn on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice. Basic Clin Neurosci. 2018;9(6):389-396.

Corda MG, Costa E, Guidotti A. Specific proconvulsant action of an imidazobenzodiazepine (RO 15-1788) on isoniazid convulsions. Neuro pharmacology. 1982;21(1):91-4.

Uzman S, Uludağ Yanaral T, Toptaş M, Koç A, Taş A, Bican G. Acute isoniazid intoxication: an uncommon cause of convulsion, coma and acidosis. Tuberk Torak. 2013;61(1):50-3

Tajender V, Saluja J. INH induced status epilepticus: response to pyridoxine. Indian Journal of Chest Diseases and Allied Sci. 2006;48(3):205-6.

Romero JA, Kuczler FJ Jr. Review Isoniazid overdose: recognition and management. Journal of American Family Physician. 1998;15;57(4):749-52.

Bhuvaneshwari. Effect of nimodipine and diclofenac in experimentally induced convulsions using INH and Electro convulsometer in rats and mice. Journal of Drug Delivery and Therapeutics. 2015; 5(1):61-64.

Akpakpan EI, Bassey UEOE, Etim Ekanemesang UM. The Effect of Ethanol Extract of Ripe Denettia tripetala Fruit (Pepper Fruit) on Indices of Liver andKidney Function in Male Albino Wistar Rats. International Journal Biochemistry Research and Review. 2017;16(3):1-7.

Arka G, Mishra A, Seth A, Maurya SK. Evaluation of anticonvulsant activity of polyherbal formulation based on ayurvedic formulation. Brihad Panchagavya Ghrita. Indian Journal of Health Sci. 2016;9:158-64.

Vogel HG, Vogel WH, editors. Drug Discovery and Evaluation: Pharma-cological Assays. 2nd Ed. New York: Springer; 2002.

Bancroft JD, Gamble M. Theory and practice of histological technique. 5th Edition. Edinburg and London: Churchill Livingstone; 2002.

Anwar F, Rajbala S, Harikesh M, Imran K, Muhammad A, Garima K, Gaurav G, Prashant K. Pharmacological role of Alstonia scholaris Leaves for its anticonvulsant and sedative action. American Journal of Phytomedicine ansd Clinical Theraupeticx. 2013;1:6478-490

Egharevba HO, Idah EA. Major compounds from the essential oil of the fruit and comparative phytochemical studies of the fruits and leaves of Dennettia tripetala found in North Central Nigeria. International Journal of Pharmacognosy and Phytochemical Research. 2015;7(6);1262-1266.

Cristina F. José GB. Luis G. Pedro, Johannes JCS (2008) Factors affecting secondary metabolite production inplants: Volatile components and essential oils Flavour and Fragrance Journal. 2008;23: 213–226.

Abubakar US, Binta IK, Amina MJ, Muhammad S, Fatima A, Ukwubile CA. A review on natural products with anticonvulsant activity. International Journal of Chemistry Studies. 2017;1(2): 27-31.

Lheureux P, Penaloza A, Gris M. Pyridoxine in clinical toxicology: Eur J Emerg Med. 2005;12:78-85.

Yarbrough, Barry E, Judy PW. Isoniazid overdose treated with high dose of pyridoxine. Annals of Emergency Medicine. 1983;12(5)303-305.

Okogun JI, Ekong DEU. Essential oil of Dennettia tripetala. Chemistry and Industry. 1969;12:71.

Henics J, Wheatley DN. Cytoplasmic vacuolation adaptation and cell death: A view on new perspective and features. Journal of Biology of the cell. 1999;91:485-498.