Antioxidant and Anti-arthritic Potential of Casuarina equisetifolia Fruit Methanolic Extract

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Santosh Kumar Vaidya
Dharmesh K. Golwala
Mitali V. Shrimanker
Darpini S. Patel
Satyajit Sahoo


Aims: Antioxidant and anti-arthritic potential of Casuarina equisetifolia fruit methanolic extract.

Place: C.U. Shah College of Pharmacy and Research, Wadhwan, Surendranagar, Gujarat, India.

Methodology: Extract was subjected to qualitative and quantitative investigation then antioxidant properties of extract was determine by two method namely Nitric Oxide free radical scavenging activity and Reducing power by FeCl3 method. Based on Phytochemical and antioxidant result anti-arthritic activity was performed on wistar rats using Complete Freund’s Adjuvant (CFA) and evaluated different parameter like paw volume, arthritic index, biochemical parameter and hematological parameter.

Results: In this study found that the methanolic extract of Casuarina equisetifolia fruit (MLCEF) contain significant percentage of secondary metabolite like poly phenol that properties was proved antioxidant activity. Antioxidant properties was determined by two methods the MLCEF IC50 Value 30.27±2.43 and 158.45±7.15 was found IC50 value reveled that MLCEF is a potent free radical scavenge capacity. Casuarina equisetifolia fruit (MLCEF) of 400 mg/kg, p.o. showed significant increasement in body wt from day 12th to 21st and significantly reduction in rat paw edema was observed from day 6th to 21st and it could be significantly normalize the haematological (like Hb, WBC and ESR) and biochemical abnormalities (like urea, ALT, AST and ALP) adjuvant induced arthritic rats in both developing and developed phases of CFA induced arthritis.         

Conclusion: The study outcome reveled that MLCEF is contain significant amount of polyphenol and that properties exhibit good antioxidant properties and significant antiarthritic properties. The actual mechanism of action of extracts on adjuvant induced arthritis is not clear with these studies. The action of extracts on proinflammatory mediators like TNF-α, Interleukins and other relevant mediators will be carried out in future to study its mechanism.

Anti-citrullinated, auto-oxidative, premature aging, TNF-α.

Article Details

How to Cite
Vaidya, S. K., Golwala, D. K., Shrimanker, M. V., Patel, D. S., & Sahoo, S. (2020). Antioxidant and Anti-arthritic Potential of Casuarina equisetifolia Fruit Methanolic Extract. European Journal of Medicinal Plants, 31(1), 42-53.
Original Research Article


Patel CJ, Tyagi S, Halligudi N, Yadav J. Antioxidant activity of herbal plants: A recent review. Journal of Drug Discovery and Therapeutics. 2013;1:01-08.

Lü JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems. J Cell Mo Med. 2010; 14:840–860.

Carocho M, Ferreira IC. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol. 2013;51:15–25.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4:118–126.

Augustyniak A, Bartosz GC. Natural and synthetic antioxidants: An updated overview. Free. Radic Res. 2010;44: 1216–1262.

Valko M, Leibfritz, D, Moncol J, Cronin MT, Mazúr M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Boil. 2007;39:44–84.

Valko M, Rhodes C, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Interact. 2006;160:1–40.

Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R. Antioxidants and human diseases. Clin Chim Acta. 2014;436:332–347.

Valavanidis A, Vlachogianni T, Fiotakis K, Loridas S. Pulmonary oxidative stress, inflammation and cancer: Respirable particulate matter, fibrous dusts and ozone as major causes of lung carcinogenesis through reactive oxygen species mechanisms. Int J Environ Res Public Heal. 2013;10:3886–3907.

Hadjigogos K. The role of free radicals in the pathogenesis of rheumatoid arthritis. Panminerva Med. 2003;45:7–13.

Wojtunik-Kulesza KA, Oniszczuk A, Oniszczuk T, Waksmundzka-Hajnos M. The influence of common free radicals and antioxidants on development of Alzheimer’s disease. Biomed Pharmacother. 2016;78:39–49.

Geto N. Anti-aging and aging factors in life. The role of free radicals. Radiat Phys Chem. 2007;76:1577–1586.

Kalla AA, Stanwix A, Gotlieb D. Rheumatoid arthritis: Clinical guideline 2003. S Afr Med J. 2003;991–1012.

Jacobson PB, Borgan SJ, Wiliox DM. A new Spin an old model in vivo evaluation of disease progress by magnetic response imaging with respect to standard inflammatory parameters and his pathology in adjuvant arthritis rat. Arthritis Rheum. 1999;42:2060-73.

Boldt AB, Goeldner I, de Messias, Reason IJ. Relevance of the lectin pathway of complement in rheumatic diseases. Adv Clin Chem. 2012;56:105–153.

Oroian M, Escriche I. Antioxidants: Characterization, natural sources, extraction and analysis. Food Res. Int. 2015;74:10–36.

Dorman H, Peltoketo A, Hiltunen R, Tikkanen M. Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem. 2003;83:255–262.

Zunino SJ, Storms DH, Stephensen CB. Diets rich in polyphenols and vitamin A inhibit the development of type I autoimmune diabetes in nonobese diabetic mice. J Nutr. 2007;137:1216.

Möller P, Loft S. Dietary antioxidants and beneficial effect on oxidatively damaged DNA. Free. Radic. Boil Med. 2006;41: 388–415.

Sikora E, Cie´slik E, Topolska K. The sources of natural antioxidants. Acta Sci. Pol. Technol. Aliment. 2008;7:5–17.

Abdollahzad H. Importance of antioxidants in rheumatoid arthritiss. Austin Arthritis. 2016;1:1005.

Gupta S, Shukla R, Sharma KK. Antidiabetic, antihypercholesterolemic and antioxidant effect of Ocimum sanctum Linn. seed oil. Ind J Exp Biol. 2006; 44:300-303.

Kokate CK, Purohit AP. Text book of Pharmacognosy. 2004;29:542.

Panchawat S, Rathore KS, Sisodia SS. A review on herbal antioxidants. Int J Pharm Tech Res. 2010;2:232-239.

Hawaiian plants and tropical flowers, Casuarina equisetifolia Common Ironwood.

Casuarina equisetifolia ironwood.

Khare CP. Indian medicinal plants an illustrated dictionary. Springer Science and Business Media, LLC. 2007;131.

Joker D. Casuarina equisetifolia L. Danida Forest seed center; 2000.

Nash RJ, Thomas PI, Waigh RD, et al. Casuarine: A very highly oxygenated pyrrolizidine alkaloid. Tet Lett. 1994;35(42):7849-7852.

Kantheti USK, Kumar DY, Ganinna B, Nath PK. Casuarina equisetifolia effect as antidiabetic and antihyperlipidemic on streptozocin induced rats with diabetes. IJCTPR. 2014;2:432-436.

Aher AN, Pal SC, Patil UK, Yadav SK. Evaluation of preliminary anticancer activity of Casuarina equisetifolia Frost (Casuarinaceae). Planta Indicia. 2008; 4:45-48.

Takahashi H, Luchi M. Fujita, Minami HY, Fukuyama Y. Coumaroyl triterpenes from Casuarina equisetifolia. Phytochemistry. 1999;51:543-550.

Hunshal CS, Channal HT, Alagawadi AR, Patil RH. Allelopathy in ecological agriculture and forestry. 2000;209-227.

Li-Hua Z, Gong-Fu Y, Yi-Ming L, Hai-Chao Z, Qi Z. Seasonal changes in tannin and nitrogen contents of Casuarina equisetifolia branchlet. J Zhejiang University Sci. 2009;10:103-119.

Mhaskar KS, Blatter E, Cauis JF. Kirtikar and Basu’s illustrated Indian Medicinal plants, 3rd Edn. Sri Satguru Publications, Delhi, India; 2009.

Maiden JH. The useful native plants of Australia. Turner and Henderson, Sydney, Australia; 1889.

Shafiq Y. Effect of light intensity on the growth of seedlings of Pinusbruta, Cupressuss empervivens and Casuarina equisetifolia. Mesopotamia J Agric. 1974; 9:73-85.

Arthur WW, Craig RE. Casuarina equisetifolia. Species Profile for Pacific Island and Agro-forestry. Permanent Agriculture Resources (PAR). 2006;1-11.

Chen XH, Hu F, Kong CH. Varietal improvement in rice allelopathy. Allelopathy J. 2008;22:379-384.

Rangari VD. Pharmacognosy & Phytochemistry. 8th ed. Nashik: Career Publication; 2008.

Harbone JR, Phytochemical methods: A guide to modern techniques of Plant Analysis. Science Paperbacks; 1984.

Golwala DK, Patel LD. Pharmacognostical studies of Bauhinia variegata Linn. Stem International J. of Pharmaceutical Res. 2012;3:127-130.

Vaidya SK, Bothara SB. Total polyphenolic content and In-vitro antioxidant potential of extracts of creeping herb Ipomoea reniformis (Roxb.) Choisy. American J Phytomed and Clin. Therapeutics. 2014; 12:1462-1469.

Nayan R. Bhalodia, Pankaj B. Nariya, Acharya RN, Shukla VJ. In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn. Ayu. 2013; 34:209–214.

Sanmugapriya E, Senthamil SP, Venkataraman S. Evaluation of antiarthritic activity of Strychnos potatorum Linn seeds in Freund’s adjuvant induced arthritic rat model. BMC Complementary and Alternative Medicine. 2010;10(56):2-9.

Ahmadi M, Zare MA, Hashemlou M, Hejazi M. Study on anti inflammatory effect of scorpion (Mesobuthus eupeus) venom in adjuvant-induced arthritis in rats. Archives of Razi Institute. 2009;64:51-56.

Kathiriya A, Das K, Kumar EP, Mathai KB, Evaluation of antitumor and antioxidant activity of Oxalis corniculata Linn. against Ehrlich Ascites Carcinoma on Mice. Iran J Cancer Prev. 2010;4:157-65.

Martın AI, Castillero E. Adipose tissue loss in adjuvant arthritis is associated with a decrease in lipogenesis, but not with an increase in lipolysis. J of Endocrinol. 2008; 197:111-119.

Briot K, Garnero P, Henanff A, Dougados M, Roux C. Body weight, body composition, and bone turnover changes in patients with spondyloarthropathy receiving anti-tumour necrosis factor a treatment. Ann Rheum Dis. 2005;64: 1137–1140.

Eswar KK, Mastan SK. Anti-arthritic property of the methanolic extract of Syzygium cumini seeds. International. J of Integ. Biol. 2008;4:56.

Colpert KM, Evidence that adjuvant arthritis in the rat is associated with chronic pain. Pain. 1987;28:201-222.

William JK. Arthritis and allied condition. A textbook of rheumatology, 3rd Edn, A Waverlay Company, Baltimore, Tokyo. 1996;1:1207.

Filho PY, et al. The urea cycle in the liver of arthritic rats. Mol Cell Biochem. 2003; 243:97-106.

Cawthorne MA, Palmer ED, Green J. Adjuvant-induced arthritis and drug-metabolizing enzymes. Biochemical Pharmacology. 1976;25:2683-2688.