Phytochemical Screening and Antioxidant Potential of Aqueous Extracts of Millettia laurenti, Lophira alata and Milicia excelsa, Commonly Used in the Cameroonian Pharmacopoeia

Main Article Content

Ferdinand L. E. Edoun
Boris R. T. Tchuente
Ruth E. K. Dibacto
Hippolyte T. Mouafo
Alex D. K. Tchuenchieu
Abomo A. C. Ndzana
Gabriel N. Medoua

Abstract

Aims: As the world nowadays is turning towards the research of biologically active natural compounds, this work aimed at assessing the antioxidant potential of compounds contained in the aqueous extracts of three common Cameroonian pharmacopoeia plants, namely Millettia laurenti (Wengé) seeds, Lophira alata (Azobé) leaves and Milicia excelsa (Iroko) barks and the associated bioactive compounds.

Methodology: After being dried and ground, they were macerated in water and the polyphenols, tannins, flavonoids, and alkaloids quantified. The antioxidant potential of the extracts was evaluated through DPPH free radical scavenging, NO scavenging, phosphomolybdate method (TAC), and iron-reducing power (FRAP).

Results: The mean concentrations obtained ranged between 527 and 1213 µg GAE/g DM for polyphenols, 0.39 and 0.65 µg GAE/g DM for tannins, 19.79 and 27.06 µg QE/g DM for flavonoids, 15.72 and 16.02 µg QuE/g DM for alkaloids. Aqueous extracts of Wengé (AE-WG) and Azobé (AE-AZ) exhibited the highest and significantly similar contents. AE-AZ presented the highest iron reducing power (0.015 µg AAE/g DM at 10 mg/mL) and NO scavenging (IC50=3.63 mg/mL) while AE-WG showed the highest DPPH scavenging activity (IC50 = 4.20 mg/mL) and total antioxidant capacity (0.39 µg AAE/g DM at 10 mg/mL). No significant correlation was observed between studied bioactive compounds and the different antioxidant responses except flavonoids and tannins with TAC (p<0.05).

Conclusion: AE-AZ and AE-WG exhibited different antioxidant mechanisms and are therefore of high interest for potential use in the food industry and medicine with reserves to toxicological studies.

Keywords:
Aqueous extracts, Wengé seeds, Azobé leaves, Iroko barks, antioxidant potential.

Article Details

How to Cite
Edoun, F. L. E., Tchuente, B. R. T., Dibacto, R. E. K., Mouafo, H. T., Tchuenchieu, A. D. K., Ndzana, A. A. C., & Medoua, G. N. (2020). Phytochemical Screening and Antioxidant Potential of Aqueous Extracts of Millettia laurenti, Lophira alata and Milicia excelsa, Commonly Used in the Cameroonian Pharmacopoeia. European Journal of Medicinal Plants, 31(11), 11-23. https://doi.org/10.9734/ejmp/2020/v31i1130295
Section
Original Research Article

References

Yadav A, Kumari R, Yadav A, Mishra JP, Srivatva S, Prabha S. Antioxidants and its functions in human body - A review. Res Environ Life Sci. 2016;9(11):1328-1331.

Sharma J, Maity A. Pomegranate phytochemicals: Nutraceutical and therapeutic values fruit, vegetable and cereal science and biotechnology. Global Science Books. 2010;4(2):56-76.

Rahman AHMM, Rahman MdM. Medicinal plants having anti-obesity potentiality available in Bangladesh: A review. Biol Med Case Rep. 2018;02(01):4-11.

Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009;2(5): 270 8.

Santos-Sánchez FN, Salas-Coronado R, Villanueva-Cañongo C, Hernández-Carlos B. Antioxidant compounds and their antioxidant mechanism. Shalaby E. Ed. Antioxidants. 2019; London.

DOI: 10.5772/intechopen.85270

Anwar H, Hussain G, Mustafa I. Antioxidants from natural sources. In: Shalaby E, Azzam GM. Ed. Antioxidants in Foods and Its Applications InTech; 2018.

Available:http://dx.doi.org/10.5772/intechopen.75961

ISBN: 978-1-83881-640-7

Allaith A. Antioxidants in date fruits and the extent of the variability of the total phenolic content: Review and analysis. Shalaby E. Ed. Antioxidants. London. 2019;1-26.

DOI: 10.5772/intechopen.83851

Konstantinidi M, Koutelidakis AE. Functional foods and bioactive compounds: A review of its possible role on weight management and obesity’s metabolic consequences. Medicines. 2019; 6(94):1-24

Available:https://doi.org/10.3390/medicines6030094.

Lourenço SC, Moldão-Martins M, Alves VD. Antioxidants of natural plant origins: From sources to food industry applications. Molecules. 2019;24(22):4132.

Available:https://doi.org/10.3390/molecules24224132.

Goodarzi S, Rafiei S, Javadi M, Haghighian HK, Noroozi S. A review on antioxidants and their health effects. Journal of Nutrition and Food Security. 2018;3 (2):106-112.

Arjmand A. Antioxidant activity of pomegranate (Punica granatum L.) polyphenols and their stability in probiotic yoghurt. Masters by Research, Applied Sciences, RMIT University. 2011;149.

Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. 2010;10(21):1-8.

Available:https://doi.org/10.1186/1472-6882-10-21

Kaurinovic B, Vastag D. Flavonoids and phenolic acids as potential natural antioxidants. In: Shalaby E. Ed. Antioxidants. 2018; London.

DOI: 10.5772/intechopen.83731

Banzouzi JT, Prost A, Rajemiarimiraho M, Ongoka P. Traditional uses of African Milletia species (Fabaceae). International Journal of Botany. 2008;4(4):406-420.

Available:https://doi.org/10.3923/ijb.2008.406.420

Biwolé AB, Bourland N, Daïnou K, Doucet J-L. Definition of the ecological profile of the azobe, Lophira alata, an African woody species of great importance: Literature review and perspectives for future research. Biotechnol. Agron. Soc. Environ. 2012;16(2):217-228.

Hsieh YHP, Ofori JA. Innovations in food technology for health. Asia Pac J Clin Nutr. 2007;16:65–73.

Singleton V, Rossi J. Colorimetry of total phenolics with phosphomolydic-phospho-tungstic acid reagents. American Journal of Enology and Viticulture. 1965;16:144-158.

Bainbridge Z, Tomlins K, Wellings K. and Westby A. Methods for assessing quality characteristics of non-grain starch staples. (Part 2. Field Methods.). Methods for assessing quality characteristics of non-grain starch staples. Natural Resources Institute, Chatham, Kent; 1996.

ISBN 0- 85954-400-1

Singh DK, Srivastava B, Sahu A. Spectrophotometric Determination of rauwolfia alkaloids: Estimation of Reserpine in Pharmaceuticals. Anal Sci. 2004;20(3):571 3.

Available:https://doi.org/10.2116/analsci.20.571

Sanchez-Moreno C, Larrauri JA, Saura-Calixto F. A procedure to measure the antiradical efficiency of polyphenols. J Sci Foods Agri. 1998;76(2):270-276.

Available:https://doi.org/10.1002/(SICI)1097-0010(199802)

Marocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide-scavenging properties of ginkgo Biloba extract EGb 761. Biochemical and Biophysical Research Communication. 1994;201(2): 748-755.

Available:https://doi.org/10.1006/bbrc.1994.1764

Oyaizu. Studies on products of browing reaction: Antioxidants activities of products of browing reaction prepared from glucosamine. Japanese journal of Nutrition and Dietetics. 1986;44:307-315.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry. 1999;269(2):337–41.

Available:https://doi.org/10.1006/abio.1999.4019

Altemimi A, Lakhssassi N, Baharlouei A, Watson D, Lightfoot D. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017;6(4):42.

Available:https://doi.org/10.3390/plants6040042

Cheok CY, Salman HAK, Sulaiman R. Extraction and quantification of saponins: A review. Food Res Int. 2014;59:16 40.

Available:https://doi.org/10.1016/j.foodres.2014.01.057

Saxena HO, Deshmukh A, Kakkar A, Singh N. Phytochemical screening and assessment of secondary metabolites in different plant parts of Solanum xanthocarpum : A Dashmool species. Indian J Trop Biodiv. 2014;22(2):164-169.

Ngoua-Meye-Misso R-L, Sima-Obiang C, Ndong JDLC, Ondo JP, Ovono AF, Obame-Engonga L-C. Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon. Egyptian Journal of Basic and Applied Sciences. 2018;5(1):80-86.

Available:https://doi.org/10.1016/ j.ejbas.2017.11.003

Teugwa MC, Sonfack DCR, Fokom R, Penlap BV, Amvam ZPH. Antifungal and antioxidant activity of crude extracts of three medicinal plants from Cameroon pharmacopea. J Med Plant Res. 2013; 7:1537-42.

Azantsa BGK, Mbong M-AA, Takuissu GR, Matsinkou RS, Djuikoo ILN, Youovop JF, Ngondi JL, Oben JE. Anti-hemolytic, Anti-lipid peroxidation and antioxidant properties of three plants locally used to treat metabolic disorders: Allium sativum, Persea americana and Citrus sinensis. J Food Res. 2019;8(4):89.

Available:https://doi.org/10.5539/jfr.v8n4p89

Sas K, Szabó E, Vécsei L. Mitochondria, Oxidative stress and the kynurenine system, with a focus on ageing and neuroprotection. Molecules. 2018;23(1):191.

Kikakedimau KNR, Musuyu MD, Nsendo MKO, Doumas P, Kahambu MS, Taba KM, Luyindula NS. Correlation between Antioxidants and Antiradical Activities with In Vitro Antimalarial Activity of Phyllanthus odontadenius. Acta Scientific Medical Sciences. 2019;3(7):144-154.

Villaño D, Fernández-Pachón MS, Moyá ML, Troncoso AM, García-Parrilla MC. Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta. 2007;71(1):230 5.

Available:https://doi.org/10.1016/j.talanta.2006.03.050

Kadum H, Hamid AA, Abas F, Ramli NS, Mohammed AKS, Muhialdin BJ, Jaafar AH. Bioactive Compounds Responsible for Antioxidant Activity of Different Varieties of Date (Phoenix dactylifera L.) Elucidated by 1 H- NMR Based Metabolomics. Int J Food Prop. 2019;22(1):462 76.

Available:https://doi.org/10.1080/10942912.2019.1590396

Valdiléia TU, Cleyton MMS, Adonias AC, Antônio EGS, Mariana HC. Free radical scavenging ability of Ximenia americana L. stem bark and leaf extracts. J Appl Pharm Sci. 2016;091 096.

Available:https://doi.org/10.7324/JAPS.2016.60213

Erol NT, Sarı F, Velioglu YS. Polyphenols, alkaloids and antioxidant activity of different grades turkish black tea. Gida. 2010;35(3):161-8.

Nithya TG, Jayanthi J, Ragunathan MG. Antioxidant activity, total phenol, flavonoid, alkaloid, tannin, and saponin contents of leaf extracts of salvinia molesta D. S. Mitchell (1972). Asian J Pharm Clin Res. 2016;9: 200-3.

Jahanban-Esfahlan A, Amarowicz R. Walnut (Juglans regia L.) shell pyroligneous acid: Chemical constituents and functional applications. RSC Adv. 2018;8(40):22376 91.

Available:https://doi.org/10.1039/C8RA03684E.

Adwas AA, Elsayed IAS, Axab AE, Quwaydir FA. Oxidative stress and antioxidant mechanisms in human body. J Appl Biotechnol Bioeng. 2019;6(1):43‒47.

Tanaka M, Chiu WK, Nagashima Y, Taguchi T. Application of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi. 1988;54(8):1409- 1414.

Available:https://doi.org/10.2331/suisan.54.1409

Stafussa AP, Maciel GM, Rampazzo V, Bona E, Makara CN, Junior BD, Haminiuk CWI. Bioactive compounds of 44 traditional and exotic Brazilian fruit pulps: phenolic compounds and antioxidant activity. Int J Food Prop. 2018;21(1):106 18.

Available:https://doi.org/10.1080/10942912.2017.14

Farahmandfar R, Kenari RE, AsnaashariM, Shahrampour D, Bakhshandeh T. Bioactive compounds, antioxidant and antimicrobial activities of Arum maculatum leaves extracts as affected by various solvents and extraction methods. Food Sci Nutr. 2019;(7):465–475.

Available:https://doi.org/10.1002/fsn3.815