In vitro Antioxidant Activities and Phytochemical Analysis of Methanol Extracts of Leaves and Stems of Lumnitzera racemosa
Firdaus Mukhtar Quraishi
Department of Life Sciences, University of Mumbai, Vidyanagari Campus, Santacruz (E), 400 098, Mumbai, India
B. L. Jadhav *
Department of Life Sciences, University of Mumbai, Vidyanagari Campus, Santacruz (E), 400 098, Mumbai, India
Neeti Kumar
Department of Life Sciences, University of Mumbai, Vidyanagari Campus, Santacruz (E), 400 098, Mumbai, India
*Author to whom correspondence should be addressed.
Abstract
Aim: To study antiradical and the reducing power activities in the leaves and stems methanol extracts of Lumnitzera racemosa mangrove species.
Design: Soxhlet extraction of leaves and stems using methanol for in vitro antioxidant assay.
Place and Duration of Study: Department of Life Sciences, University of Mumbai, Vidyanagari Campus, Santacruz (East), Mumbai, India, April, 2014 to December, 2014
Methodology: The plant branches were collected in the month of May, identified by an expert taxonomist. The leaves and stems were separated, washed, shed dried, powdered and Soxhlet methanol extracts were prepared to study antioxidant properties using DPPH (1, 1-diphenyl-2-picrylhydrazyl) Scavenging and Reducing power assay.
Results: Both DPPH test and reducing power assay showed better antioxidant activity in leaves than stems. The scavenging activity in the leaves was (23.31 μg/mL) while in stems (111.5 μg/mL) as compared to ascorbic acid (14.98 μg/mL)
Conclusion: Overall L. racemosa has shown antioxidant properties in which leaves were more potent than stems.
Keywords: Lumnitzera racemosa, antioxidant, DPPH, reducing power, scavenging activity