Anti-urease Activity of Mimusops elengi Linn (Sapotaceae)
Hina Zahid *
Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, 75270, Pakistan
Ghazala H. Rizwani
Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, 75270, Pakistan
Arfa Kamil
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-qbal, Karachi-75300, Pakistan
Huma Shareef
Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, 75270, Pakistan
Sabiha Tasleem
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-qbal, Karachi-75300, Pakistan
Ajmal Khan
H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
*Author to whom correspondence should be addressed.
Abstract
Aim: Mimusops elengi L. (Sapotaceae) commonly known as Bakul is a small to large evergreen tree found all over the different parts of the world. Literature survey showed no reports on the anti urease activity of the flowers and leaves of Mimusops elengi. The present study was therefore envisaged to evaluate the anti urease activity of the methanolic extracts (MFE and MLE) of M. elengi.
Study Design: Assessment of anti urease activity and total phenolic contents.
Methodology: For the determination of total phenolic content of M. elengi extracts (MFE and MLE) the reaction mixture contained 50% Folin-Ciocalteu reagent (0.5 mL), 20% (w/v) sodium carbonate solution (2.5 mL), and gallic acid solution (1, 2.5, 5, 10, 25, 50, 100 mg/ml) or sample extracts (1.0 mL). Urease inhibition activity was determined by mixing Urease (Jack bean) method was employed for urease inhibition activity.
Results: From the standard calibration curve of gallic acid the total phenolic contents in extracts (MFE and MLE) was found to be 7.5±0.28 mg/g, 96.8±4.6 mg/g. Urease inhibitory activity of M. elengi extracts (MFE and MLE) was comparable with standard Thiourea. The percentage urease inhibition of Thiourea was found to be 98.2%, IC50 88.2 ± 0.01 mg/ml. The maximum urease inhibitory activity was shown by the leaves extract (MLE) i. e, 77.9% (IC50 62.1±1.20 mg/ml) while methanolic extract of flower (MFE) produced 47.6% urease inhibition activity. The total phenolic contents in both methanolic extracts (MFE and MLE) was found to be 7.5±0.28 mg/g, 96.8±4.6 mg/g respectively.
Conclusion: It is, therefore, concluded that this plant can be a sources to isolate some natural urease inhibitory agents.
Keywords: Mimusops elengi Linn, H. pylori, urease inhibition, total phenolic content