Evaluation of Acute Toxicity and Hepatoprotective Activity of Scrofoloso - 5 (S5) and Livome, Electro Homoeopathic Herbal Preparations against CCl4 Induced Liver Toxicity
P. Suresh Babu *
Deparment of Biochemistry, Arogyadhama Institute of Para Medical Sciences and Clinical Research, Shankaraghatta – 577 451, Affiliated to Para Medical Boad Govt. of Karnataka, India
V. Krishna
Phytochemistry and Pharmacology Laboratory, P.G. Department of Studies and Research in Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Shankaraghatta – 577 451, Karnataka, India
Ajith Singh
Department of Phytochemistry, Kings Herbals, Jalander, Punjab, India
Ramesh Babu
Department of Pathology Shimoga Institute of Medical Sciences and Research Shimogaga-577201, Karnataka, India
Venkatesh .
Phytochemistry and Pharmacology Laboratory, P.G. Department of Studies and Research in Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Shankaraghatta – 577 451, Karnataka, India
K. Pradeep
Phytochemistry and Pharmacology Laboratory, P.G. Department of Studies and Research in Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Shankaraghatta – 577 451, Karnataka, India
*Author to whom correspondence should be addressed.
Abstract
Aim: Many medical professionals are using electro homoeopathic preparations which are available in Indian market. In the present study, the electro homoeopathic medicines Scrofoloso 5 (S5) and Livome were evaluated for their acute toxicity and hepatoprotective activity against CCl4 induced toxicity.
Study Design: For acute toxicity studies six groups with six mice each one was used to evaluate two test samples. Group I,II and III were administered test sample S5 in the dose 0.25ml,0.5ml and 1.0ml respectively and Group IV,V and VI were administered test sample Livome with the dose 0.25ml,0.5ml and 1.0ml respectively. Behaviors were studied up to 14 days. For hepatoprotective evaluation 5 groups of six rats Group I, II III, IV and V were employed and biochemical and histological profile were studied.
Materials and Methods: The electro homoeopathic preparations of Scrofoloso-5 (S5) and Livome were purchased from the local market. The acute toxicity was evaluated in female swiss albino mice, while the hepatoprotective efficacy was screened in male Wistar rats. The liver function biochemical parameters and the histopathological profile were used as criteria for hepatoprotective estimation.
Results: There was no acute toxicity for both S5 and Livome drugs up to high dose of 1.0 ml/day for mice of 25g b.wt.orally. The hepatoprotective activity of S5 was similar to the standard hepatoprotective drug silymarin and Livome effects are more significant than the standard hepatoprotective drug silymarin. The histological profile of the liver tissue showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the controls.
Conclusion: The Electro homoeopathic medicines available in market for the Electro homoeopathic practitioners S5 and Livome afforded significant protection from CCl4 induced liver damage.
Keywords: Electro homoeopathy, acute toxicity, hepatoprotective, scrofoloso 5, livome