Hepatoprotective Activity of Holostemma ada Kodien shcult, Extract against Paracetamol Induced Hepatic Damage in Rats
Junapudi Sunil *
Department of Pharmacy, Research Scholar, Jawaharlal Nehru Technological University Kakinada, Kakinada, Andhra Pradesh, - 533 003, India and Department of Pharmaceutical Chemistry, Geethanjali College of Pharmacy, Jawaharlal Nehru Technological University Hyderabad, Cheryaal, Keesara, Ranga Reddy district, Andhra Pradesh, 501301, India
Janapati Yasodha Krishna
Department of Pharmaceutical Chemistry, Sri Sarada College of Pharmacy, Jawaharlal Nehru Technological University Hyderabad, Ananthram (vil), Bhongiri (M), Nalgonda (Dt), Andhra Pradesh, -508116, India
Pallaval Veera Bramhachari
Department of Biotechnology, Krishna University, Machilipatnam, Krishna (Dt), Andhra Pradesh, -521001, India
*Author to whom correspondence should be addressed.
Abstract
Aim: To study the hepatoprotective activity of alcoholic extract of Holostemma ada Kodien Shcult against paracetamol (PCM) induced liver damage in rats.
Place and Duration of Study: Department of pharmaceutical chemistry and department of pharmacology Geethanjali College of Pharmacy, Cherryal, Keesara, Ranga Reddy District, Andhra Pradesh, India during June 2012 and Jan 2014.
Methods: Hepatotoxicity was induced by paracetamol (PCM) and various biochemical parameters were assessed to confirm the induced degrees of hepatoprotoxicity. The various extracts were tested on animals and various biochemical parameters such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP) and total serum bilirubin (SB) were investigated the hepatoprotective activity was also confirmed by histopathological studies of normal and hepatodamage and post treatment studies with the extract.
The Results: Pre-treatment of the rats with alcoholic extract prior to paracetamol (PCM) administration caused a significant reduction in the values of AST, ALT, ALP and SB (p<0.01) approximately comparable to the hepatoprotective of standard drug was silymarin and confirmed by histopathological examination of the liver tissue of control and treated animals.
Conclusion: From the results it can be concluded that Holostemma ada Kodien Shcult possesses the hepatoprotective effect against paracetamol (PCM) induced liver damage in rats.
Keywords: Holostemma ada kodien shcult, hepatoprotective activity, paracetamol, albino rats, histopathological studies