HPLC Determination of the Eight Constitutes in Portulaca oleracea L. from Different Locations
Junjun Ai
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 116600 Dalian, China and First Affiliated Hospital of Dalian Medical University, 116011, China
Aijing Leng *
First Affiliated Hospital of Dalian Medical University, 116011, China
Xiaomei Gao
School of Pharmacy, Beijing University of Traditional Chinese Medicine, 100102 Beijing, China
Wenjie Zhang
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 116600 Dalian, China
Di Li
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 116600 Dalian, China
Liang Xu
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 116600 Dalian, China
Xixiang Ying
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 116600 Dalian, China
*Author to whom correspondence should be addressed.
Abstract
Aims: The objective of the present research was to simultaneously determine the eight constituents in Portulaca oleracea L. from different locations by a sensitive and specific high-performance liquid chromatographic (HPLC) method, including caffeic acid (CA), p-coumaric acid (p-CA), scopoletin (SCOP), ferulic acid (FA), quercetin-3-O-rhamnoside (QR), quercetin (QUER), apigenin (APIG) and bergapten (BERG) using rhein as the internal standard.
Methodology: With gradient elution procedure and mobile phase A (0.1% formic acid) and B (acetonitrile) ratio linear changing, the wavelength was set to 320 nm to determinate the eight constituents.
Result: The method validation presented good accuracy with recoveries in the range of 93.67-113.3% and good precision with RSD values less than 12.1%.The results indicated that there existed the significant differences of the eight constituents in P. oleracea L. from 20 different locations.
Conclusion: The results can be used to reasonably assess the drug quality and exploit P. oleracea L.
Keywords: High performance liquid chromatography, Portulaca oleracea L., determination, different locations