Hepatoprotective Effect of Origanum elongatum against Carbon Tetrachloride (CCl4) Induced Toxicity in Rats
B. Douhri *
Laboratory of Biology and health, Team biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
M. Idaomar
Laboratory of Biology and health, Team biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
N. Skali Senhaji
Laboratory of Biology and health, Team biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
A. Ennabili
National Institute of Medicinal and Aromatic Plants, University Sidi Mohamed Ben Abdellah, BP 159, Taounate principal, 34000, Morocco
J. Abrini
Laboratory of Biology and health, Team biotechnology and applied Microbiology, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetuan, Morocco
*Author to whom correspondence should be addressed.
Abstract
Aims: This study was undertaken to investigate the potential effect of methanol extract of Origanum elongatum (OEME) given orally by gavage against intraperitoneal injection a single dose of Carbon tetrachloride (CCl4)(0.6 ml/kg) induced hepatotoxicity in rats.
Study Design: Biochemical analysis, histological examination and in vivo study.
Place and Duration of Study: Laboratory of Biology and health (Faculty of Science), between June 2012 and August 2012.
Methodology: Hepatoprotective activity of OEME at four doses of 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg body weight. The degree of protection was estimated by biochemical analysis of serum liver biomarkers: AST, ALT, ALP and by liver histopathological examination.
Results: The total phenolic content of OEME (83.61 ± 0.19 mg AGE / g extract) and total flavonoid content (10.85 ± 0.05 mg QE / g extract) were found significantly high. The present study showed that OEME was dose dependently decreased hepatic histopathological changes and serum liver biomarkers levels in CCl4 intoxicated rats.
Conclusion: Data from present results revealed the hepatoprotection of OEME against hepatotoxic products or drugs.
Keywords: Origanum elongatum, silymarin, hepatoprotection, CCl4, hepatotoxicity, serum liver biomarkers, histopathological examination