Syzygium sp (Myrtaceae) Extracts: Inhibition of Alpha Amylase
Tuanny Cavatão de Freitas
Laboratório de Bioquímica, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triangulo Mineiro – UFTM, Zipe Code 38015.050, Uberaba, MG, Brazil
Chrystian Araújo Pereira *
Laboratório de Bioquímica, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triangulo Mineiro – UFTM, Zipe Code 38015.050, Uberaba, MG, Brazil
Luciana Lopes Silva Pereira
Laboratório de Bioquímica, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triangulo Mineiro – UFTM, Zipe Code 38015.050, Uberaba, MG, Brazil
*Author to whom correspondence should be addressed.
Abstract
Aims: The aims of this study were: a) prepare crude extracts of commercial teas Syzygium sp by different methods (maceration, decoction and infusion) in different proportions (1:20, 1:50 and 1:100) and b) submit them the inhibition assays digestive enzyme alpha-amylase before and after exposure to a simulated gastric fluid.
Place and Duration of Study: Laboratório de Bioquímica, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Brazil, between August 2012 and July 2013.
Methodology: Five samples of Syzygium sp teas were purchased commercially from pharmacies, drugstores and health food stores.
Results: In the first step - inhibition of enzymatic extracts of the teas without simulated gastric fluid - the results showed significant inhibition of alpha-amylase (mean 92.84%, CV = 3.86%). However, when these extracts were placed in contact with the simulated gastric fluid was observed decreased inhibition of alpha – amylase, suggesting a possible reduction of hypoglycemic potential of these teas under physiological conditions.
Conclusion: Aqueous extract Syzygium sp have high percentages of inhibition of the enzyme amylase, suggesting a potential hypoglycemic effect. Additional studies aiming at elucidating the chemical compounds present in the extracts of Syzygium sp using chromatographic techniques will be performed.
Keywords: Syzygium, diabetes, amylase, gastric fluid