Evaluation of the Effects of Aqueous Leaves Extract of Cnestis ferruginea from Côte d’Ivoire on Male Rat Reproductive System
Zougrou N’guessan Ernest *
Laboratoire d’Endocrinologie et Biologie de la Reproduction, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Kouassi Kouadio Aubin
UFR Science de la Nature, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
Tahiri Annick
Laboratoire d’Endocrinologie et Biologie de la Reproduction, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Blahi Adélaïde Nadia Méa
Laboratoire d’Endocrinologie et Biologie de la Reproduction, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Kouakou Koffi
Laboratoire d’Endocrinologie et Biologie de la Reproduction, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
*Author to whom correspondence should be addressed.
Abstract
Against growing male infertility in the world, investigations are undertaken to find new bioactive molecules. Thus, the aim of this study was to evaluate the pharmacological effects of aqueous extract of Cnestis ferruginea on the reproductive parameters of male rats. Indeed, 36 male rats were divided into 2 groups of 18 each and treated for 30 days (set I) and 60 days (set II). Each set was subdivided into three groups. Group 1 (control) received distilled water. Groups 2 and 3 were treated with 50 (AECF50) and 100 (AECF100) mg/kg body weight of the aqueous extract of Cnestis ferruginea respectively. The results showed that extract induces significant increase in the wet weight of testis, seminal vesicles, epididymis, prostate and levator ani muscle as well as the dry weight of the latter. On the sperm parameters, the extract produced a significant increase in the number of motile spermatozoa, number of spermatozoa and number of normal spermatozoa. The extract also increased serum levels of pituitary gonadotropins (FSH and LH) and testosterone. Histological study showed that, Cnestis ferruginea induced significant increase in the seminiferous tubules diameter. In conclusion, the extract of Cnestis ferruginea could contain androgen-like substances capable of improving the fertility of male rats.
Keywords: Cnestis ferruginea, testis, spermatozoa, gonadotrophins, testosterone