Comparative Study on Dpph Free Radical Scavenging Activity of 25 Kinds of Traditional Chinese Medicinal Plants
LI Pei-Xia
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
HE Dong-Hao
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
GONG Meng-Yu
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Shun Yuan-Feng
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
LI Meng-Lin
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Huang Yi *
School of life science and engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
*Author to whom correspondence should be addressed.
Abstract
Aims: To determine and compare the antioxidant activity of water and ethanol extract of 25 kinds of traditional chinese medicinal plants.
Results: The ethanol extract of 4 kinds of medicinal herbs had the strongest scavenging activity. They were Magnolia officinalis, Rheum officinale, Psoralea corylifolia and Radix Bupleuri. In addtion, Rheum laciniatum, Chrysanthemum morifolium, Magnolia officinalis and Salvia miltiorrhiza had the strongest scavenging activity of their water extract. On the basis of the above comparison, we evaluated EC50 and total phenolic content of their ethanol extract. The EC50 of Magnolia officinalis, Rheum officinale, Psoralea corylifolia and Radix Bupleuri were 2.75mg·mL-1, 11.82mg·mL-1, 25.22mg·mL-1and 42.67mg·mL-1. The total phenlic content of them were 4.80μg·L-1 , 1.19μg·L-1, 1.07μg·L-1 and 0.75μg·L-1, respectively.
Conclusion: The results showed the correlation between the antioxidant activity and the total phenol content. Furthermore, the reaction time of the DPPH test affected the free radical scavenging, which reflected the difference of the extract component would impact the test method.
Keywords: Antioxidant activity, DPPH; EC50, total phenol content