A Study on Phytochemical and Anticancer Activities of Epiphytic Orchid Aerides odorata Lour.
Jhansi Katta
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, India.
Venkatesh Rampilla
Dr. L.H.R Government Degree College, Mylavaram, Krishna District, Andhra Pradesh, India.
S. M. Khasim *
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study was carried out to evaluate the phytochemical composition and anticancer activities of leaf extract of Aerides odorata Lour., a widely distributed epiphytic herb found in the Eastern Ghats of Vizianagram district.
Methodology: The solvents like n-hexane, ethyl acetate and methanol were used to extract dried leaf material of A. odorata. These extracts were analysed for phytochemical constituents by GC-MS analysis and in vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay.
Results: Preliminary phytochemical analysis revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, and terpenoids. GC-MS analysis determines presence of 15 compounds in ethyl acetate and 14 compounds in methanol extracts respectively. Among two extracts a total 13 compounds have anticancer activity. Both the solvent extracts exhibit significant cancer cell growth inhibition with IC50 value ranging between 26.211 µg/mL to 59.061 µg/mL.
Conclusion: Methanol about the best solvent and its activity. Our result showed A. odorata is a promising source of anticancer drugs.
Keywords: GC-MS analysis, anticancer, Aerides odorata