In vitro Antioxidant and Anti-inflammatory Activities of the Flower Extracts of Hibiscus vitifolius L
D. Prabhakaran *
Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India and Chettinad Cement Corporation Ltd., Ariyalur, Tamil Nadu, India.
A. Rajeshkanna
Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
M. M. Senthamilselvi
Kamarajar Government Arts College, Surandai, Tamil Nadu, India.
S. Solomon
Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Objective: To determine the antioxidant and anti-inflammatory activities of the solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L.
Methods: The flower extract assessed for antioxidant activity using the 1,1–diphenyl-2-picryl-hydrazile (DPPH) radical scavenging assay and the reduced power assay was performed using the Ferric Reducing Capacity (FRC) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) to test the production method of nitric oxide (NO).
Results: The solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L showed good antioxidant activity in the scavenging DPPH radicals and the FRC assay compared to the standard sample. This powder sample also showed good anti-inflammatory activity in cell viability (LPS induced PBMC) assay and nitric oxide (NO) assay.
Conclusion: These results suggest that the powder sample extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L has substantial antioxidant and anti-inflammatory activity.
Keywords: Antioxidant, solid powder, Hibiscus vitifolius, PBMC.