Anti-oxidant and Anti-inflammatory Activity of Ethyl Acetate Fraction of Moringa oleifera Flowers
A. Rajeshkanna *
Department of Chemistry, Ananda College, Devakottai, Tamil Nadu, India and Department of Chemistry, Periyar E.V.R. College, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
M. M. Senthamilselvi
Department of Chemistry, Periyar E.V.R. College, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India and Government Arts College, Surandai, Tirunelveli, Tamil Nadu, India.
D. Prabhakaran
Department of Chemistry, Periyar E.V.R. College, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India and Chettinad Cement Corporation Ltd., Ariyalur, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To investigate the antioxidant and anti-inflammatory activities of ethyl acetate extract of Moringa oleifera flowers.
Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between April 2017 and January 2018.
Methodology: Extraction and fractionation were carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The anti-inflammatory effect of the extract was investigated by HRBC membrane stabilization and Albumin denaturation methods. Anti-oxidant effect of the extract was determined by DPPH assay and ABTS method.
Results: The dry sample extracted from the ethyl acetate fraction of Moringa oleifera flowers possess highly anti-oxidant activity showed by the DPPH assay and ABTS method and also having anti-inflammatory activity is determined by human red blood cell (HRBC) membrane stabilization and Albumin denaturation methods. However, these effects need to be confirmed using in vivo models and clinical trials before its utilization as a therapeutic agent.
Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Moringa oleifera flowers possesses effective anti-oxidant and anti-inflammatory activities.
Keywords: ABTS assay, anti-inflammatory activity, antioxidant activity, HRBC method, Moringa oleifera.