Antioxidant and Antimutagenic (Anticlastogenic) Activity of Alcoholic Extract of Bauhinia variegata (Linn.) Root
Dharmesh K. Golwala *
Shankersinh Vaghela Bapu Institute of Pharmacy, Vasan, Gandhinagar, Gujarat, India.
Santosh Kumar Vaidya
Shankersinh Vaghela Bapu Institute of Pharmacy, Vasan, Gandhinagar, Gujarat, India.
Kishor K. Dholwani
Laxminaryan Dev College of Pharmacy, Bharuch, Gujarat, India.
Darpini S. Patel
K.B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat, India.
Satyajit Sahoo
C. U. Shah College of Pharmacy and Research, Wadhwan, Gujarat, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: Antioxidant and Antimutagenic (Anticlastogenic) activity of alcoholic extract of Bauhinia variegata (Linn.) root.
Place: C. U. Shah College of Pharmacy and Research, Wadhwan, Surendranagar, Gujarat, India.
Methodology: Shade dried Bauhinia variegata (Linn.) root, extraction was carryout by isolation extract were subjected to primary and secondary Phytochemical investigation. Then In-vitro antioxidant properties were estimated by reducing power and nitric oxide free radical scavenging method. Based on Phytochemical constituent and antioxidant properties In-vivo Antimutagenic (Anticlastogenic) activity was performed.
Results: Preliminary phytochemical investigation revealed the presence of carbohydrates, free amino acids, and secondary metabolites like tannins, phenolic compounds and flavonoids, then polyphenol estimation found ALBV contains 86.38% phenolic compounds. In antioxidant properties determination IC50 respectively found 55.27±2.57 µg/ml, 125.52±8.15 µg/ml against of Ascorbic acid and Curcumin. Then In-vivo Antimutagenic (Anticlastogenic) activity ALBV shows significant reeducation in % MNPCE, % MNNCE and P/N ratio at 24 h, 48 h and 72 h against the cyclophosphamide-induced mutagenicity.
Conclusion: Therefore, from the present study, it is concluded that alcoholic extract of Bauhinia variegata root (ALBV) can prove to be a very good antioxidant and effective chemopreventive against cyclophosphamide-induced mutagenesis.
Keywords: Bauhinia variegata root, anticlastogenic, polychromatic erythrocytes, micronucleated polychromatic erythrocytes, micronucleated normochromatic erythrocytes.