In-vitro Anti-lipoxygenase and Membrane Stabilization Activity of Crude Extracts of Euphorbia lateriflora (Schum and Thonn)
Olasunkanmi Adedoyin A. *
Department of Science Laboratory Technology, Federal Polytechnic, Ilaro, Ogun State, Nigeria.
Fadahunsi Olumide Samuel
Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria and Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Olorunnisola Olubukola Sinbad
Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study was aimed at evaluating the in-vitro anti-inflammatory potential of crude extracts of Euphorbia lateriflora.
Place of Study: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Methodology: The in-vitro anti-inflammatory activity of aqueous and ethanol extracts of E. lateriflora were evaluated via anti-lipoxygenase and red cell membrane stabilization assays using spectrophotometric technique.
Results: The aqueous and ethanol extracts demonstrated significant anti-lipoxygenase activity with the aqueous extracts (IC50: 0.34 mg/ml) and the ethanol extracts (IC50: 0.25 mg/ml), showing comparable activity with standard indomethacin (0.27 mg/ml). Contrary to what was observed in the anti-lipoxygenase study, the aqueous extract demonstrated a better membrane stabilization activity with IC50 (1.13 mg/ml) than the ethanol extracts (5.44 mg/ml). The standard diclofenac drug had an IC50 of 1.02 mg/kg. Both the aqueous and the ethanol extracts demonstrated significant anti-inflammatory activity.
Conclusion: Observation from this study established the considerable anti-inflammatory potential of this plant by inhibiting lipoxygenase activity and stabilization the membrane of the red blood cells.
Keywords: E. lateriflora, crude extracts, anti-lipoxygenase, membrane stabilization, anti-inflammation