In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Canthium coromandelicum Leaves
S. Thiripura Salini *
Department of Chemistry, A.V.V.M Sri Pushpam College (Affiliated to Bharahidasan University), Poondi, Thanjavur, Tamil Nadu, India.
S. Shankar
Department of Chemistry, A.V.V.M Sri Pushpam College (Affiliated to Bharahidasan University), Poondi, Thanjavur, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Oxidative stress plays a major role in diabetic complications. The study aim was to investigate the in-vitro antidiabetic and antioxidant activities of methanolic extract of Canthium coromandelicum leaves. The plant material was extracted with methanol and the methanolic extract was screened for in-vitro antioxidant activity using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) assay. The efficiency of the antidiabetic activity of the plant extract was evaluated against α-amylase and α-glucosidase digestive enzymes. The study revealed that the C. coromandelicum extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 31.52 ± 0.42 and 41.49 ± 0.28 µg/mL respectively and compared with standard acarbose drug. The extract efficiently scavenging DPPH radical with IC50 values of 65.46 ± 0.50 µg/ml. Therefore, the extract could be a promising therapeutic in management of diabetic complications.
Keywords: In-vitro anti-diabetic, antioxidant, Canthium coromandelicum, DPPH, α- amylase.