Effect of SanQiTongShuan on Rats in Stroke Recovery Period
Chunhua Hao
State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, PR China
Weiting Wang
State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, PR China
Rui Zhang
State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, PR China
Xianghua Zhang
Kunming Institute of Nephrology, Kunming 650000, PR China
Feng Guo
Kunming Institute of Nephrology, Kunming 650000, PR China
Zhuanyou Zhao
State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, PR China
Lida Tang *
Kunming Institute of Nephrology, Kunming 650000, PR China
*Author to whom correspondence should be addressed.
Abstract
Objective: This study aims to investigate the therapeutic effect and possible mechanisms of Chinese Medicine SanQiTongShuan(SQTS) on rats in stroke recovery period.
Methods: The cerebral ischemia stroke recovery period model was successfully induced by FeCl3 after the fourth day and balance beam test≤4. A total of 70 rats were used in experiments. 60 model rats were divided into six groups (n=10). The model group was administered 0.5% carboxymethylcellulose sodium (CMC), the treated groups were administered SQTS (0.5, 1, 2 g /kg), and the positive control groups were administered Naodesheng (NDS, 1.24 g /kg) and Vinpocetine (VP, 1.55 mg/kg). In addition, the other 10 rats served as a sham group and were administered 0.5% CMC. Rats in the each group were treated each day last for 30 days orally with the volume of 10 ml/kg. The motor function of beam-walking test and forelimb muscle strength were performed before the occlusion and lasted for 10, 20 and 30 d after administration. The serum inflammatory factors IL-1β, IL-6, TNFα, ICAM-1 and VCAM were measured by assay kits. In addition, the optical microscopic examination of the serial sections of impact areas was performed.
Results: The ischemic rats displayed signs of brain damage on motor function, forelimb muscle strength and histopathology. SQTS (0.5, 1.0, 2.0 g /kg) restored the beam-walking scores by 14.8% (P>0.05), 22.2%(P>0.05), 37%(P<0.05) after 20 d treatment and 24.0%(P>0.05), 48.0%(P<0.05), 40.0%(P<0.05) after 30 d treatment; increased the strength by 39.0%(P>0.05), 45.6%(P>0.05), 54.0%(P<0.05) after 20 d treatment and 40.3%(P>0.05), 43.7%(P<0.05), 54.5%(P<0.05) after 30 d treatment. On the other hand, the histological changes were less severe and the inflammatory factors IL-6, TNFα, VCAM were decreased at the different degree, with respect to the model group.
Conclusion: We proposed that SQTS, a promising Traditional Chinese Medicine Patent Prescription, can be used as a therapeutic agent for stroke recovery period via alleviating inflammatory responses.
Keywords: SanQiTongShuan, stroke recovery period, rats, motor function, inflammatory factors