Evaluation of Antibacterial, Anti-Oxidant and Cytotoxic Activity of Organic Extracts of Mahogany Seeds
. Assaduzzaman
Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Bangladesh.
Md. Ziaul Amin
Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Bangladesh.
Mohammad Habibur Rahman
Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Bangladesh.
Md. Rasel Uddin
Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Bangladesh.
Md. Shohanuzzaman
Environmental Science and Disaster Management, Bangabandhu Sheikh Mujibur Rahman Science & Technology University, Bangladesh.
Pritylata Mandal
Department of Biotechnology, North South University, Dhaka, Bangladesh.
Bristy Amy Karmoker
Department of Microbiology, Stamford University, Dhaka, Bangladesh.
Md. Raihan Dipu
Department of Microbiology, Stamford University, Dhaka, Bangladesh.
Md. Rashed Nejum *
Department of Biotechnology and Genetic Engineering, Islamic University, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
The perception of normal medicine is changing, and the use of traditional or herbal medicine increasing worldwide due to our harmonious nature of the biological system. Many parts of the plants possess an impressive array of medicinal benefits, mahogany seed is one of them. The aim of this study is to evaluate the antimicrobial, anti-oxidant and cytotoxic activity of organic extracts of mahogany seeds. For determination of antimicrobial and cytotoxic activity disk diffusion and brine shrimp lethality bioassay were used. DPPH free radical test were performed for evaluation of antioxidant activity. Ethanol, n-hexane and aqueous extracts were examined with the total number of 10 bacterial strain. Here, ethanol extract showed higher antimicrobial activity than n-hexane and aqueous extract for both gram positive and negative bacteria. In brine shrimp lethality bioassay LC50 values were 82 μg/ml, 95 μg/ml and 93 μg/ml for ethanol, n-hexane and aqueous extracts respectively. The scavenging DPPH free radicals were found to be concentration dependent. The LC50 value was measured at 140µg/ml, 160µg/ml and 180µg/ml for ethanol, n-hexane and aqueous extract. The results of this study indicated that the mahogany seeds can be used as an easily accessible source of natural antioxidants and Cytotoxic compounds which might be helpful in preventing the progress of various oxidative stresses and cell culture assay.
Keywords: Antibacterial, anti-oxidant, shrimp, anti-inflammatory, ethnopharmacological profile, organic extracts, microbiology, antimicrobial activity.