Sabicea brasiliensis Wernham: Antioxidant Activity, Proliferative Effect and Modulation of Vascular Adenine Nucleotides Metabolism
Douglas Souza Oliveira
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Mikaelle Costa Correia
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Bruna Juber de Araújo
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Fernanda Cardoso da Silva
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Paula Marynella Alves Pereira Lima
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Marcos de Souza Gomes
Instituto de Química, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Thaise Gonçalves de Araújo
Instituto de Biotecnologia, Universidade Federal de Uberlândia - Campus Patos de Minas, Minas Gerais, Brazil.
Cristina Ribas Fürstenau *
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, São Paulo, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Aims: Study addressed the antioxidant activity (AA) of Sabicea brasiliensis roots crude extract (CE), ethyl acetate (EAF), and hydro-methanolic (HMF) fractions, and its impact on cell viability and adenine nucleotide hydrolysis in vascular A7r5 cells.
Materials and Methods: AA of CE, EAF and HMF were determined by the inhibition of the DPPH and ABTS radicals. Total phenolic content was evaluated by Folin-Ciocalteau. Cell viability was determined by MTT assay at different concentrations (62.5; 125; 250 and 500 μg·mL-1) of EAF and HMF after 24, 48 and 72 h. Ectonucleotidase activities were evaluated by colorimetric methods after 48 h EAF or HMF treatment.
Results: The highest AA was observed for CE (76%), followed by EAF (46%) and HMF (23%). Phenolic content followed the same pattern. After 48 h, EAF increased A7r5 vascular cells viability by 40%, 40%, 62% and 25% at distinct concentrations, respectively; while HMF augmented it by 50% (500 μg·mL−1). Finally, after 48 h EAF (500 μg·mL−1) decreased about 50% of ATP and ADP metabolism while HMF inhibited 56 and 59% the hydrolysis of NPP substrate (at 125 and 250 μg·mL−1).
Conclusion: Study confirmed the high AA of S. brasiliensis, which influences vascular cells proliferation and purines metabolism, pointing to potential cellular pathways that may support the popular use of this plant.
Keywords: Brazilian savanna, blood of Christ, antioxidant activity, proliferative effect, purines metabolism, vascular smooth muscle cells