Diuretic, Kaliuretic and Anti-Natriuretic Properties of Aqueous Extract of Celosia trigyna L. (Amaranthaceae) on Wistar Rats
Touwindséda Aimée Sawadogo
Laboratoire de Physiologie Animale, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR SVT), Université Joseph Ki-Zerbo, 03 BP 7021, Ouaga 03, Burkina Faso.
Youssoufou Ouedrogo *
Laboratoire de Physiologie Animale, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR SVT), Université Joseph Ki-Zerbo, 03 BP 7021, Ouaga 03, Burkina Faso.
Lazare Belemnaba
Département de Médecine et Pharmacopée Traditionnelles et Pharmacie, Institut de Recherche en Sciences de la Santé, Centre National de la Recherche Scientifique et Technologique (MEPHATRA PH/IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
Filkpièrè Léonard Da
University Norbert Zongo of Koudougou, BP: 376, Burkina Faso.
Balé Bayala
Laboratoire de Physiologie Animale, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR SVT), Université Joseph Ki-Zerbo, 03 BP 7021, Ouaga 03, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aims: The purpose of this study was to determine the diuretic and electrolyte excretion properties of the aqueous extract of Celosia trigyna L. (Amaranthaceae) on female Wistar rats.
Methodology: The extraction of active principles was done by macerating aerial parts of the plant. The administration of the extract and other products was done by single-dose gavage. Measurements of urinary flow rate (UFR), natriuria, kaliuria and chloruria were taken on urine collected for twenty-four hours after each product was administered. Diuretic activity (DA) and diuretic index (DI), natriuretic and saluretic effects, and carbonic anhydrase inhibition were calculated. A NaCl solution (0.9%) was used as a negative control; furosemide and aldactone were respectively used as hypokalemic and hyperkalemic positive controls.
Results: We observed a significant increase in UFR, confirmed by the values of DA and DI, obtained after the administration of extract. For electrolyte excretion, we observed an increase of the kaliuria (p ˂ 0.001) and a decrease of natriuria (p ˂ 0.001) after the extract was administered; chloruria did not significantly changed. We also found a drastic anti-natriuretic dose-dependent effect while saluretic activity and carbonic anhydrase inhibition were not clearly observable.
Conclusion: These results confirm the ethnobotanical data about diuretic effect of Celosia trigyna L. extract. This diuretic effect would be supported by a specific increase in K+ excretion suggesting that the extract is possibly hypokalemic. The anti-natriuretic effect suggests that extract possess an aldosterone-like properties.
Keywords: Celosia trigyna, furosemide, aldactone, diuretic, natriuretic, kaliuretic.